Metabolic
Engineering
Metabolic engineering is a purposeful modification of intermediary metabolism
using recombinant DNA technology, and is aimed at using microrganisms for industrial
production prosesses. One of the major challenges when optimizing bacteria for
industrial purposes is to identify enzymes controlling metabolic bottlenecks
leading to the synthesis of the desired metabolite. Of particular interest in
our research has been to experimentally study the metabolic effects of manipulations
of the kinetic properties and expression levels of an enzyme controlling a key-step
in carbon metabolism. Some of the vectors described in the
Gene
Expression section has been very useful in this respect.
Key Papers
Winther-Larsen, H. C., Blatny, J. M., Valand, B., Brautaset, T., and Valla, S. (2000). Pm promoter expression mutants and their use in broad-host-range RK2 plasmid vectors. Metabolic Engineering , Vol.2, No.2, pp. 92-103.
Brautaset, T., Petersen, S.B., and Valla, S. (2000). In vitro determined kinetic properties of mutant phosphoglucomutases, and their effects on sugar catabolism in Escherichia coli . In press Metabolic Engineering .
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Biopolymers
Bioprospecting
of marine microorganisms
Biosyntesis
of L-Lysin from Methanol
Engineered
antibiotic synthesis
Gene
Expression
Osmoregulation
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