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last update: February 27, 2004
Metabolic Engineering

Metabolic engineering is a purposeful modification of intermediary metabolism using recombinant DNA technology, and is aimed at using microrganisms for industrial production prosesses. One of the major challenges when optimizing bacteria for industrial purposes is to identify enzymes controlling metabolic bottlenecks leading to the synthesis of the desired metabolite. Of particular interest in our research has been to experimentally study the metabolic effects of manipulations of the kinetic properties and expression levels of an enzyme controlling a key-step in carbon metabolism. Some of the vectors described in the Gene Expression section has been very useful in this respect.

Key Papers


Winther-Larsen, H. C., Blatny, J. M., Valand, B., Brautaset, T., and Valla, S. (2000). Pm promoter expression mutants and their use in broad-host-range RK2 plasmid vectors. Metabolic Engineering , Vol.2, No.2, pp. 92-103.

Brautaset, T., Petersen, S.B., and Valla, S. (2000). In vitro determined kinetic properties of mutant phosphoglucomutases, and their effects on sugar catabolism in Escherichia coli . In press Metabolic Engineering .


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Biopolymers
Bioprospecting of marine microorganisms
Biosyntesis of L-Lysin from Methanol
Engineered antibiotic synthesis
Gene Expression
Osmoregulation



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