Publications from the Department of Biotechnology can be seen
here
.
For abstracts please click on the publications.
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| 2010
|
Lale, R., Berg, L., Stüttgen, F., Netzer, R., Stafsnes, M., Brautaset, T., Aune, T.E.V., Valla. S.
Continuous control of the flow in biochemical pathways through 5'-UTR sequence modifications in mRNA expressed from the broad-host-range Pm promoter
Appl. Environ. Microbiol.
Epub ahead of print.
|
| 2010
|
Engelhardt, K., Degnes, K. F., Kemmler, M., Bredholt, H., Fjærvik, E., Klinkenberg, G., Sletta, H., Ellingsen, T. E. and Zotchev, S. B.
Production of a new thiopeptide antibiotic, TP-1161, by a marine Nocardiopsis species.
Appl. Environ. Microbiol.
76, (15), 4969-4976.
|
| 2010
|
Engelhardt, K., Degnes, K. F. and Zotchev, S. B.
Isolation and Characterization of the Biosynthetic Gene Cluster for Thiopeptide Antibiotic TP-1161.
Appl. Environ. Microbiol.
Epub ahead of print.
|
| 2010
|
Tøndervik, A., Klinkenberg, G., Aarstad, O. A., Drabløs, F., Ertesvåg, H., Ellingsen, T. E., Skjåk-Bræk, G., Valla, S. and Sletta, H.
Isolation of mutant alginate lyases with cleavage specificity for di-guluronic acid linkages.
J. Biol. Chem.
in press.
|
| 2010
|
Stafsnes, M. H., Josefsen, K. D., Kildahl-Andersen, G., Valla, S. and Ellingsen, T. E. and Bruheim, P.
Isolation and characterization of marine pigmented bacteria from Norwegian coastal waters and screening for carotenoids with UVA-blue light absorbing properties.
The Journal of Microbiology
48,(1), 16-23.
|
| 2010
|
Aune, T. E. V., Bakke, I., Drabløs, F., Lale, R., Brautaset, T. and Valla, S.
Directed evolution of the transcription factor XylS for development of improved expression systems.
Microb. Biotechnol.
3, (1), 38–47.
|
| 2010
|
Jørgensen, H., Degnes, K. F., Dikiy, A., Fjærvik, E., Klinkenberg, G. and Zotchev, S. B.
Insights into the evolution of macrolactam biosynthesis through cloning and comparative analysis of the biosynthetic gene cluster for a novel macrocyclic lactam, ML-449.
Appl. Environ. Microbiol.
76, (1), 283–293.
|
| 2009
|
Aune, T. E. V. and Aachmann, F. L.
Methodologies to increase the transformation efficiencies and the range of bacteria that can be transformed.
Appl. Microbiol. Biotechnol.
13 p.
|
| 2009
|
Hakvåg S, Fjaervik E, Klinkenberg G, Borgos SE, Josefsen KD, Ellingsen
TE, Zotchev SB.
Violacein-producing Collimonas sp. from the sea surface microlayer of
costal waters in Trøndelag, Norway.
Mar Drugs.
12;7(4):576-88.
|
| 2009
|
Preobrazhenskaya MN, Olsufyeva EN, Tevyashova AN, Printsevskaya SS,
Solovieva SE, Reznikova MI, Trenin AS, Galatenko OA, Treshalin ID,
Pereverzeva ER, Mirchink EP, Zotchev SB.
Synthesis and study of the antifungal activity of new mono- and
disubstituted derivatives of a genetically engineered polyene antibiotic
28,29-didehydronystatin A1 (S44HP).
J Antibiot (Tokyo).
2010 Feb;63(2):55-64.
|
| 2009
|
Aakvik, T., Degnes, K. F., Dahlsrud, R., Schmidt, F., Dam, R., Yu, L., Völker, U., Ellingsen, T. E. and Valla, S.
A plasmid RK2-based broad-host-range cloning vector useful for transfer of metagenomic libraries to a variety of bacterial species.
FEMS Microbiology Letters
296,149-158.
|
| 2009
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Ertesvåg, H., Valla, S. and Skjåk-Bræk, G.
Enzymatic Alginate Modification.
In Alginates: Biology and Applications. Edited by Rehm, B. H. A. Springer-Verlag.
95-115.
|
| 2009
|
Aachmann, F. L. and Vee Aune, T. E.
Use of cyclodextrin and its derivatives for increased transformation efficiency of competent bacterial cells.
Appl. Microbiol. Biotechnol.
83, 589–596.
|
| 2009
|
Gimmestad, M., Ertesvåg, H., Heggeset, T. M. B., Aarstad, O., Svanem, B. I. G., and Valla, S.
Characterization of three new Azotobacter vinelandii alginate lyases, one of which is involved in cyst germination.
J. Bacteriol.
doi:10.1128/JB.00455-09.
|
| 2009
|
Setubal, J. C. et al. (Ertesvåg H, Valla S, Mærk M,)
The genome sequence of Azotobacter vinelandii, an obligate aerobe specialized to support diverse anaerobic metabolic processes .
J. Bacteriol.
Ahead of print.
|
| 2009
|
Jørgensen, H., Degnes, K. F., Sletta, H., Fjærvik, E., Dikiy, A., Herfindal, L., Bruheim, P., Klinkenberg, G., Bredholt, H., Nygård, G., Døskeland, S. O., Ellingsen, T. E. and Zotchev, S. B.
Biosynthesis of macrolactam BE-14106 involves two distinct PKS systems and amino acid processing enzymes for generation of the aminoacyl starter unit.
Chemistry & Biology
16, 1109-1121.
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| 2009
|
Jørgensen, H., Fjærvik, E., Hakvåg, S., Bruheim, P., Bredholt, H., Klinkenberg, G., Ellingsen, T. E. and Zotchev, S. B.
Candicidin biosynthesis gene cluster is widely distributed among Streptomyces spp. isolated from the sediments and the neuston layer of the Trondheim fjord, Norway.
Appl. Environ. Microbiol.
75(10), 3296–330.
|
| 2009
|
Berg, L., Lale, R., Bakke, I., Burroughs, N. and Valla, S.
The expression of recombinant genes in Escherichia coli can be strongly stimulated at the transcript production level by mutating the DNA-region corresponding to the 5'-untranslated part of mRNA.
Microb. Biotechnol.
2(3), 379-389.
|
| 2009
|
Zotchev, S. B. and Caffrey, P.
Genetic analysis of nystatin and amphotericin biosynthesis.
Methods in Enzymology
Vol.459, Ch.11, 243-258.
|
| 2009
|
Valla, S.
The future is artificial.
Microb. Biotechnol.
2(2), 152-153.
|
| 2009
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Bakke, I., Berg, L., Aune, T. E. V., Brautaset, T., Sletta, H., Tøndervik, A. and Valla, S.
Random mutagenesis of the Pm promoter as a powerful strategy for improvement of recombinant-gene expression.
Appl. Environ. Microbiol.
75(7), 2002–2011.
|
| 2009
|
Zotchev, Sergey B.
Antibiotics: Biosynthesis.
Wiley Encyclopedia of Chemical Biology.
1-9.
|
| 2009
|
Jakobsen,Ø.M., Brautaset,T., Degnes,K.F., Heggeset,T.M.B., Balzer,S., Flickinger,M.C.,Valla,S. and Ellingsen,T.E.
Overexpression of wild-type aspartokinase increases L-lysine production in the thermotolerant methylotrophic bacterium Bacillus methanolicus.
Appl. Environ. Microbiol.
75(3), 652-661.
|
| 2009
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Ballance, S., Aarstad, O. A., Aachmann, F. L., Skjåk-Bræk, G. and Christensen, B. E.
Preparation of high purity monodisperse oligosaccharides derived from mannuronan by size-exclusion chromatography followed by semi-preparative high-performance anion-exchange chromatography with pulsed amperometric detection.
Carbohydr. Res.
344, 255-259.
|
| 2009
|
Preobrazhenskaya, M. N., Olsufyeva, E. N., Solovieva, S. E., Tevyashova, A. N., Reznikova, M. I., Luzikov, Y. N., Terekhova, L. P., Trenin, A. S., Galatenko, O. A., Treshalin, I. D., Mirchink, E. P., Bukhman, V. M., Sletta, H. and Zotchev, S. B.
Chemical modification and biological evaluation of new semisynthetic derivatives of 28,29-didehydronystatin A1 (S44HP), a genetically engineered antifungal polyene macrolide antibiotic.
Journal of Medicinal Chemistry
52, 189-196.
|
| 2009
|
Brautaset, T., Lale, R., and Valla, S.
Positively regulated bacterial expression systems.
Microb. Biotechnol.
2(1), 15-30
|
| 2008
|
Bar, N. S. and Lale, R.
Modeling and control of the protein synthesis process in eukaryotic cells.
47th IEEE Proceedings: Conference on Decision and Control (CDC)
179-184.
|
| 2008
|
Valla, S., Ertesvåg, H., Tonouchi, N. and Fjærvik, E.
Bacterial cellulose production: Biosynthesis and applications. In Microbial production of biopolymers and polymer precursors. Edited by Rehm, B. H. A. Caister Academic Press.
43-78.
|
| 2008
|
Hakvåg, S., Fjærvik, E., Josefsen, K. D., Ian, E., Ellingsen, T. E. and Zotchev, S. B.
Characterization of
Streptomyces
spp. isolated from the sea surface microlayer in the Trondheim fjord, Norway.
Mar. Drugs
6, 620-635.
|
| 2008
|
Brautaset, T., Sletta, H., Nedal, A., Borgos, S. E. F., Degnes, K. F., Bakke, I., Volokhan, O., Sekurova, O. N., Treshalin, I. D., Mirchink, E. P., Dikiy, A., Ellingsen, T. E. and Zotchev, S. B.
Improved antifungal polyene macrolides via engineering of the nystatin biosynthetic genes in
Streptomyces noursei
.
Chemistry & Biology
15, 1198–1206.
|
| 2008
|
Aachmann, F. L. and Skjåk-Bræk, G.
1H, 15N, 13C resonance assignment of the AlgE6R1 subunit from the Azotobacter vinelandii mannuronan C5-epimerase.
Biomol NMR Assign, elektronisk versjon.
3p.
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| 2008
|
Rozeboom, H. J., Bjerkan, T. M., Kalk, K. H., Ertesvåg, H., Holtan, S., Aachmann, F. L., Valla, S. and Dijkstra, B. W.
Structural and mutational characterization of the catalytic A-module of the mannuronan C-5-epimerase AlgE4 from
Azotobacter vinelandii. J. Biol. Chem.
283,(35), 23819–23828.
|
| 2008
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Jakobsen,A.N., Aasen,I.M., Josefsen,K.D. and Strøm,A.R.
Accumulation of docosahexaenoic acid-rich lipid in thraustochytrid Aurantiochytrium sp. strain T66: effects of N and P starvation and O2 limitation.
Appl. Microbiol. Biotechnol.
80,297–306.
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| 2008
|
Caffrey,P., Aparicio, J.F., Malpartida,F. and Zotchev,S.B.
Biosynthetic engineering of polyene macrolides towards generation of improved antifungal and antiparasitic agents.
Current Topics in Medicinal Chemistry
8, 639-653.
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| 2008
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Steigedal, M., Sletta, H., Moreno, S., Mærk, M., Christensen, B. E., Bjerkan, T. M., Ellingsen, T. E., Espìn, G., Ertesvåg, H. and Valla, S.
The Azotobacter vinelandii AlgE mannuronan C-5-epimerase family is essential for the in vivo control of alginate monomer composition and for functional cyst formation.
Environmental Microbiology
10,(7), 1760–1770.
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| 2008
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Steigedal, M. and Valla, S.
The Acinetobacter sp. chnB promoter together with its cognate positive regulator ChnR is an attractive new candidate for metabolic engineering applications in bacteria.
Met. Eng.
10, 121-129
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| 2008
|
Bredholt, H., Fjærvik, E., Johnsen, G. and Zotchev, S. B.
Actinomycetes from sediments in the Trondheim Fjord, Norway: Diversity and biological activity.
Mar. Drugs
6, (1), 12-24.
|
| 2007
|
Nedal A, Sletta H, Brautaset T, Borgos SE, Sekurova ON, Ellingsen TE,
Zotchev SB.
Analysis of the mycosamine biosynthesis and attachment genes in the
nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455.
Appl. Environ. Microbiol.
73, 22, 7400-7407.
|
| 2007
|
Bredholdt, H., Galatenko, O. A., Engelhardt, K., Fjærvik, E., Terekhova, L. P. and Zotchev, S. B.
Rare actinomycete bacteria from the shallow water sediments of the Trondheim fjord, Norway: isolation, diversity and biological activity.
Environ. Microbiol.
9,
(11),
2756–2764.
|
| 2007
|
Brautaset, T., Jakobsen, Ø. M., Josefsen, K. D., Flickinger, M. C. and Ellingsen, T. E.
Bacillus methanolicus
: a candidate for industrial production of amino acids from methanol at 50°C.
Appl. Microbiol. Biotechnol.
74, 22–34.
|
| 2007
|
Sletta, H., Tøndervik, A., Hakvåg, S., Aune, T. E. V., Nedal, A., Aune, R., Evensen, G., Valla, S., Ellingsen, T. E. and Brautaset, T.
The presence of N-terminal secretion signal sequences leads to strong stimulation of the total expression levels of three tested medically important proteins during high-cell-density cultivations of
Escherichia coli
.
Appl. Environ. Microbiol.
73
,(3),
906–912.
|
| 2007
|
Throne-Holst, M., Wentzel, A., Ellingsen, T. E., Kotlar, H. K. and Zotchev, S. B.
Identification of novel genes involved in long-chain n-alkane degradation by Acinetobacter sp. strain DSM 17874.
Appl. Environ. Microbiol.
73
,(10),
3327–3332.
|
| 2007
|
Tøndervik, A. and Strøm, A. R.
Membrane topology and mutational analysis of the osmotically activated BetT choline transporter of
Escherichia coli
.
Microbiol.
153
,(3),
803–813.
|
| 2007
|
Wentzel, A., Ellingsen, T. E., Kotlar, H.-K., Zotchev, S. B. and Throne-Holst, M.
Bacterial metabolism of long-chain
n
-alkanes.
Appl. Microbiol. Biotechnol.
|
| 2007
|
Brautaset, T., Jakobsen, Ø. M., Josefsen, K. D., Flickinger, M. C. and Ellingsen, T. E.
Bacillus methanolicus
: a candidate for industrial production of amino acids from methanol at 50°C.
Appl. Microbiol. Biotechnol.
74, 22–34.
|
| 2006
|
Borgos
SE, Tsan P, Sletta H, Ellingsen TE, Lancelin JM, Zotchev SB. Probing the
Structure-Function Relationship of Polyene Macrolides: Engineered Biosynthesis
of Soluble Nystatin Analogues.
J Med Chem
. Apr 20;49(8):2431-2439.
|
| 2006
|
Zotchev
SB, Stepanchikova AV, Sergeyko AP, Sobolev BN, Filimonov DA, Poroikov
VV. Rational design of macrolides by virtual screening of combinatorial
libraries generated through in silico manipulation of polyketide synthases.
J Med Chem
. Mar 23;49(6):2077-87.
|
| 2006
|
Volokhan
O, Sletta H, Ellingsen TE, Zotchev SB.Characterization of the P450 Monooxygenase
NysL, Responsible for C-10 Hydroxylation during Biosynthesis of the Polyene
Macrolide Antibiotic Nystatin in
Streptomyces noursei
.
Appl Environ Microbio
l. Apr;72(4):2514-9.
|
| 2006
|
Throne-Holst
M, Markussen S, Winnberg A, Ellingsen TE, Kotlar HK, Zotchev SB. Utilization
of n-alkanes by a newly isolated strain of
Acinetobacter venetianus
:
the role of two AlkB-type alkane hydroxylases.
Appl Microbiol Biotechnol
.
Mar 7; [Epub ahead of print]
|
| 2006
|
Aachmann
FL, Svanem BI, Guntert P, Petersen SB, Valla S, Wimmer R.
NMR structure of the R-module -A parallel beta -roll subunit from an
Azotobacter
vinelandii
mannuronan C-5 epimerase.
J Biol Chem
. 2006 Jan
3; [Epub ahead of print]
|
| 2006
|
Borgos
SEF, Sletta H, Fjærvik E, Brautaset T, Ellingsen TE, Gulliksen O-M,
Zotchev SB. Effect of glucose limitation and specific mutations in the
module 5 enoyl reductase domains in the nystatin and amphotericin polyketide
synthases on polyene macrolide biosynthesis.
Arch. Microbiol
.
2006 Jan 14; [Epub ahead of print]
|
| 2005
|
Bakkevig
K, Sletta H, Gimmestad M, Aune R, Ertesvag H, Degnes K, Christensen BE,
Ellingsen TE, Valla S.
Abstract Role of the
Pseudomonas fluorescens
alginate lyase (AlgL)
in clearing the periplasm of alginates not exported to the extracellular
environment.
J Bacteriol
. 2005 Dec;187(24):8375-84.
|
| 2005
|
Sletta
H, Borgos SE, Bruheim P, Sekurova ON, Grasdalen H, Aune R, Ellingsen TE,
Zotchev SB. Nystatin biosynthesis and transport: nysH and nysG genes encoding
a putative ABC transporter system in
Streptomyces noursei
ATCC
11455 are required for efficient conversion of 10-deoxynystatin to nystatin.
Antimicrob Agents Chemother
. 2005 Nov;49(11):4576-83.
|
| 2005
|
Volokhan
O, Sletta H, Sekurova ON, Ellingsen TE, Zotchev SB. An unexpected role
for the putative 4'-phosphopantetheinyl transferase-encoding gene nysF
in the regulation of nystatin biosynthesis in
Streptomyces noursei
ATCC 11455.
FEMS Microbiol Lett
. Aug 1;249(1):57-64.
|
| 2005
|
Fjaervik
E, Zotchev SB. Biosynthesis of the polyene macrolide antibiotic nystatin
in
Streptomyces noursei
.
Appl Microbiol Biotechnol
.
Jun;67(4):436-43.
|
| 2005
|
Aachmann
FL, Svanem BG, Valla S, Petersen SB, Wimmer R. NMR assignment of the R-module
from the
Azotobacter vinelandii
Mannuronan C5-epimerase AlgE4.
J Biomol NMR
. Mar;31(3):259.
|
| 2004
|
Robles-Price
A, Wong TY, Sletta H, Valla S, Schiller NL. AlgX is a periplasmic protein
required for alginate biosynthesis in
Pseudomonas aeruginosa
.
J Bacteriol
. Nov;186(21):7369-77.
|
| 2004
|
Bjerkan
TM, Lillehov BE, Strand WI, Skjak-Braek G, Valla S, Ertesvag H. Construction
and analyses of hybrid
Azotobacter vinelandii
mannuronan C-5
epimerases with new epimerization pattern characteristics.
Biochem
J.
Aug 1;381(Pt 3):813-21.
|
| 2004
|
Sletta
H, Nedal A, Aune TE, Hellebust H, Hakvag S, Aune R, Ellingsen TE, Valla
S, Brautaset T. (2004). Broad-Host-Range Plasmid pJB658 Can Be Used for
Industrial-Level Production of a Secreted Host-Toxic Single-Chain Antibody
Fragment in
Escherichia coli
.
Appl Environ Microbiol
.
Dec;70(12):7033-9.
|
| 2004
|
Bruheim
P, Borgos SE, Tsan P, Sletta H, Ellingsen TE, Lancelin JM, Zotchev SB.
(2004).Chemical Diversity of Polyene Macrolides Produced by
Streptomyces
noursei
ATCC 11455 and Recombinant Strain ERD44 with Genetically
Altered Polyketide Synthase NysC.
Antimicrob Agents Chemother
.
Nov;48(11):4120-9.
|
| 2004
|
Bjerkan
TM, Bender CL, Ertesvag H, Drablos F, Fakhr MK, Preston LA, Skjak-Braek
G, Valla S. (2004). The
Pseudomonas syringae
Genome Encodes a
Combined Mannuronan C-5-epimerase and
O
-Acetylhydrolase, Which
Strongly Enhances the Predicted Gel-forming Properties of Alginates.
J
Biol Chem
.Jul 9;279(28):28920-9.
|
| 2004
|
Sekurova,
O.N., Brautaset, T., Sletta, H. Borgos, S.E.F., Jakobsen, Ø.M.,
Ellingsen, T.E., Strøm, A.R., Valla, S., Zotchev, S.B. (2004).
In vivo analysis of the regulatory genes in the nystatin biosynthetic
gene cluster of
Streptomyces noursei
ATCC 11455 reveals their
differential control over antibiotic biosynthesis.
J. Bacteriol.
186: 1345-1354.
|
| 2004
|
Nedal
A, Zotchev SB. (2004). Biosynthesis of deoxyaminosugars in antibiotic-producing
bacteria.
Appl Microbiol Biotechnol
. 64: 7–15.
|
| 2004
|
Brautaset
T, Jakobsen M OM, Flickinger MC, Valla S, Ellingsen TE.(2004). Plasmid-dependent
methylotrophy in thermotolerant
Bacillus methanolicus
.
J
Bacteriol
. Mar;186(5):1229-38.
|
| 2004
|
Bjerkan
TM, Lillehov BE, Strand WI, Skjak-Braek G, Valla S, Ertesvag H. (2004).
Construction and analyses of hybrid
Azotobacter vinelandii
mannuronan
C-5-epimerases with new epimerisation pattern characteristics.
Biochem
J.
381(3):813-821.
|
| 2003
|
Aachmann,
F.L., Otzen, D.E., Larsen, K.L., and Wimmer, E. (2003). Structural background
of cyclodextrin-protein interactions.
Protein Engineering, Design
and Selection
. 16: 905-912.
|
| 2003
|
Zotchev
SB. Polyene Macrolide antibiotics and their applications in human therapy.
Curr Med Chem
. Feb;10(3):211-23.
|
| 2003
|
Blatny
JM, Ertesvag H, Nes IF, Valla S.(2003). Heterologous gene expression in
Lactococcus lactis
; expression of the
Azotobacter vinelandii
algE6 gene product displaying mannuronan C-5 epimerase activity.
FEMS
Microbiol Lett
. 2003 Oct 24;227(2):229-35. PMID: 14592713.
|
| 2003
|
Gimmestad
M, Sletta H, Ertesvag H, Bakkevig K, Jain S, Suh SJ, Skjak-Braek G, Ellingsen
TE, Ohman DE, Valla S. (2003). The
Pseudomonas fluorescens
AlgG
protein, but not its mannuronan C-5-Epimerase activity, is needed for
alginate polymer formation.
J Bacteriol
. Jun 15;185(12):3515-3523.
|
| 2003
|
Jain,
S., Franklin, M.J., Ertesvåg, H., Valla, S., and Ohman, D.E. (2003).
The dual roles of AlgG in C-5-epimerization and secretion of alginate
polymers in
Pseudomonas aeruginosa
.
Mol. Microbiol
. 47:
1123-1133.
|
| 2003
|
Gimmestad
M, Sletta H, Ertesvag H, Bakkevig K, Jain S, Suh SJ, Skjak-Braek G, Ellingsen
TE, Ohman DE, Valla S. (2003). The
Pseudomonas fluorescens
AlgG
protein, but not its mannuronan C-5-Epimerase activity, is needed for
alginate polymer formation.
J Bacteriol
. Jun 15;185(12):3515-3523.
|
| 2003
|
Brautaset
T, Borgos SE, Sletta H, Ellingsen TE, Zotchev SB. Site-specific mutagenesis
and domain substitutions in the loading module of the nystatin polyketide
synthase, and their effects on nystatin biosynthesis in
Streptomyces
noursei
.
J Biol
Chem
. (2003). Apr 25;278(17):14913-9.
|
| 2003
|
Aparicio
JF, Caffrey P, Gil JA, Zotchev SB. Polyene antibiotic biosynthesis gene
clusters.
Appl
Microbiol Biotechnol
. 61(3):179-188.
|
| 2002
|
Hartmann,
M., Duun, A.S., Markussen, S., Grasdalen, H., Valla, S., and Skjåk-Bræk,
G. (2002). Time-resolved 1H and 13C NMR spectroscopy for detailed analyses
of the
Azotobacter vinelandii
mannuronan C-5-epimerase reaction.
Biochem. Biophys. Acta
. 1570: 104-112.
|
| 2002
|
Brautaset,
T., Bruheim, P., Sletta, H., Hagen, L., Ellingsen, T.E., Strøm,
A.R., Valla, S., Zotchev, S.B. (2002). Hexaene derivatives of the polyene
antifungal antibiotic nystatin produced as a result of an induced rearrangement
within the nysC polyketide synthase gene in
Streptomyces noursei
ATCC 11455.
Chem. Biol. 9:367-373.
|
| 2002
|
Wimmer
R, Aachmann FL, Larsen KL, Petersen SB (2002).NMR diffusion as a novel
tool for measuring the association constant between cyclodextrin and guest
molecules.
Carbohydr Res
. 2002 Apr 30;337(9):841-9.
|
| 2002
|
Otzen
DE, Knudsen BR, Aachmann F, Larsen KL, Wimmer R (2002). Structural basis
for cyclodextrins' suppression of human growth hormone aggregation.
Protein
Sci
. 2002 Jul;11(7):1779-87.
|
| 2001
|
Valla
S, Li J, Ertesvag H, Barbeyron T, Lindahl U. Hexuronyl C5-epimerases in
alginate and glycosaminoglycan biosynthesis.
Biochimie. 2001 Aug;83(8):819-30.
Review.
PMID: 11530215
|
| 2001
|
Santos,
P.M., Bartolo, I.D., Blatny, J.M., Zennaro, E., and Valla, S. (2001) New
broad-host-range promoter probe vectors based on the plasmid RK2 replicon.
FEMS Microbiol. Lett. 195: 91-96.
|
| 2001
|
Svanem
BI, Strand WI, Ertesvag H, Skjak-Braek G, Hartmann M, Barbeyron T, Valla
S. (2001)
The catalytic activities of the bifunctional
Azotobacter vinelandii
mannuronan C-5-epimerase and alginate lyase AlgE7 probably originate from
the same active site in the enzyme.
J Biol Chem. 2001, 276(34):31542-50
.
|
| 2001
|
Svanem,
B.I.G., Strand, W.I., Skjåk-Bræk, G., Ertesvåg, H., Hartmann, M., Bjerkan,
T., and Valla, S. (2001) The catalytic activity of the bifunctional
Azotobacter
vinelandii
mannuronan C-5-epimerase and alginate lyase AlgE7 probably
originate from the same active site in the enzyme.
J. Biol. Chem
.
|
| 2000
|
Santos,
P.M., Blatny, J.M., Bartolo, I.D., Valla, S., and Zennaro, E. (2000) Physiological
analysis of the expression of the styrene degradation gene cluster in
Pseudomonas fluorescens ST.
Appl. Env. Microbiol. 66: 1305-1310.
|
| 2000
|
Brautaset
T, Sekurova ON, Sletta H, Ellingsen TE, Strøm AR, Valla S, Zotchev
SB. (2000) Biosynthesis of the polyene antifungal antibiotic nystatin
in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction
of the biosynthetic pathway.
Chem Biol. 2000 Jun;7(6):395-403.
PMID: 10873841; UI: 20334850
|
| 2000
|
Brautaset
T, Petersen SB, Valla S. (2000) In vitro determined kinetic properties
of mutant phosphoglucomutases and their effects on sugar catabolism in
Escherichia coli.
Metab Eng. 2000 Apr;2(2):104-14
. PMID: 10935726;
UI: 20388795
|
| 2000
|
Winther-Larsen
HC, Josefsen KD, Brautaset T, Valla S. (2000) Parameters affecting gene
expression from the Pm promoter in gram-negative bacteria.
Metab Eng.
2000 Apr;2(2):79-91.
PMID: 10935724; UI: 20388793
|
| 2000
|
Draget,
K.I., Strand, B., Hartmann, M., Valla, S., Smidsrød, O., and Skjåk-Bræk,
G. (2000). Ionic and gel formation of epimerised alginates: the effect
of AlgE4.
Int. J. Biol. Macromol. 27: 117-122.
|
| 2000
|
Winther-Larsen
HC, Blatny JM, Valand B, Brautaset T, Valla S. (2000) Pm promoter expression
mutants and their use in broad-host-range RK2 plasmid vectors.
Metab
Eng. 2000 Apr;2(2):92-103.
PMID: 10935725; UI: 20388794
|
| 2000
|
Høidal,
H., Svanem, B.I., Gimmestad, M., and Valla, S. (2000) Mannuronan C-5-
epimerases and cellular differentitation of Azotobacter vinelandii.
Env.
Microbiol. 2: 27-38.
|
| 2000
|
Zotchev
S, Haugan K, Sekurova O, Sletta H, Ellingsen TE, Valla S. (2000) Identification
of a gene cluster for antibacterial polyketide-derived antibiotic biosynthesis
in the nystatin producer Streptomyces noursei ATCC 11455.
Microbiology.
2000 Mar;146 ( Pt 3):611-9.
PMID: 10746764; UI: 20208553
|
| 2000
|
Zotchev
S.B., Protopopova M., Selivanova G. (2000). p53 C-terminal interaction
with DNA ends and gaps has opposing effect on specific DNA binding by
the core.
Nucl Acids Res
28: 4005-4012.
|
| 2000
|
Garcia
De Castro A, Bredholt H, Strom AR, Tunnacliffe A. (2000) Anhydrobiotic
engineering of gram-negative bacteria.
Appl Environ Microbiol.
2000, 66(9):4142-4.
|
| 1999
|
Svanem
BI, Skjak-Braek G, Ertesvag H, Valla S. (1999) Cloning and expression
of three new Aazotobacter vinelandii genes closely related to a previously
described gene family encoding mannuronan C-5-epimerases.
J Bacteriol.
1999 Jan;181(1):68-77.
PMID: 9864314; UI: 99084941
|
| 1999
|
Ertesvag
H, Valla S. (1999) The A modules of the Azotobacter vinelandii mannuronan-C-5-epimerase
AlgE1 are sufficient for both epimerization and binding of Ca2+.
J
Bacteriol. 1999 May;181(10):3033-8
. PMID: 10322003; UI: 99255526
|
| 1999
|
Hoidal
HK, Ertesvag H, Skjak-Braek G, Stokke BT, Valla S. (1999) The recombinant
Azotobacter vinelandii mannuronan C-5-epimerase AlgE4 epimerizes alginate
by a nonrandom attack mechanism.
J Biol Chem. 1999 Apr 30;274(18):12316-22.
PMID: 10212201; UI: 99230244
|
| 1999
|
Ramstad, M.V., Ellingsen, T., Josefsen, K.,
Høidal, H.K., Valla, S., Skjåk-Bræk, G., and Levine, D.W. (1999) Properties
and action pattern of the recombinant mannuronan C-5-epimerase AlgE2.
Enzyme and Microb. Technol. 24: 636-646.
|
| 1999
|
Ertesvag
H, Hoidal HK, Schjerven H, Svanem BI, Valla S. (1999) Mannuronan C-5-epimerases
and their application for in vitro and in vivo design of new alginates
useful in biotechnology.
Metab Eng. 1999 Jul;1(3):262-9
.PMID: 10937941;
UI:20391728
|
| 1999
|
Karunakaran
P, Endresen DT, Ertesvag H, Blatny JM, Valla S . (1999) A small derivative
of the broad-host-range plasmid RK2 which can be switched from a replicating
to a non-replicating state as a response to an externally added inducer.
FEMS
Microbiol Lett. 1999 Nov 15;180(2):221-7.
PMID: 10556715;
UI: 20026136
|
| 1999
|
Sekurova
O, Sletta H, Ellingsen TE, Valla S, Zotchev S. (1999) Molecular cloning
and analysis of a pleiotropic regulatory gene locus from the nystatin
producer Streptomyces noursei ATCC11455.
FEMS Microbiol Lett. 1999
Aug 15;177(2):297-304.
PMID: 10474196; UI: 99403404
|
| 1998
|
Valla S. (1998). Broad-host-range plasmids and
their role in gene transfer in nature.
APMIS Suppl. 1998;84:19-24.
Review. No abstract available. PMID: 9850677; UI: 99067663
|
| 1998
|
Ertesvåg, H., and Valla, S.
(1998) Bacterial alginate synthesis and the mannuronan C-5-epimerase system
in Azotobacter vinelandii.
Proceedings from The International Symposium
on Biochemical Principles and Mechanisms of Biosynthesis and Biodegradation
of Polymers, Munster, June 3-6. Wiley, VCH, pp. 176-184.
|
| 1998
|
Ertesvag
H, Hoidal HK, Skjak-Braek G, Valla S. (1998) The Azotobacter vinelandii
mannuronan C-5-epimerase AlgE1 consists of two separate catalytic domains.
J Biol Chem. 1998 Nov 20;273(47):30927-32.
PMID: 9812987; UI: 99030352
|
| 1998
|
Valla, S. (1998). Broad-host-range
plasmids and their role in gene transfer in nature. APMIS Suppl. 84: 19-24.
|
| 1998
|
Ertesvag
H, Erlien F, Skjak-Braek G, Rehm BH, Valla S. (1998) Biochemical properties
and substrate specificities of a recombinantly produced Azotobacter vinelandii
alginate lyase.
J Bacteriol. 1998 Aug;180(15):3779-84.
PMID: 9683471;
UI: 98348436
|
| 1998
|
Ertesvåg, H., and Valla, S.
(1998) Biosynthesis and applications of alginates.
Polymer Degr. and
Stabil. 59: 85-91.
|
| 1998
|
Strøm, A. (1998) Osmoregulation in the
model organism Escherichia Coli: genes governing the synthesis of glycine
betaine and trehalose and their use in metabolic engineering of stress
tolerance.
J. Biosoc. Sci. 23, 437-445
|
| 1998
|
Brautaset
T, Petersen S, Valla S. (1998) An experimental study on carbon flow in
Escherichia coli as a function of kinetic properties and expression levels
of the enzyme phosphoglucomutase.
Biotechnol Bioeng. 1998 Apr 20-May
5;58(2-3):299-302.
PMID: 10191405; UI: 99208564
|
| 1998
|
Karunakaran
P, Blatny JM, Ertesvag H, Valla S. (1998) Species-dependent phenotypes
of replication-temperature-sensitive trfA mutants of plasmid RK2: a codon-neutral
base substitution stimulates temperature sensitivity by leading to reduced
levels of trfA expression.
J Bacteriol. 1998 Aug;180(15):3793-8.
PMID: 9683473; UI: 98348438
|
| 1997
|
Blatny
JM, Brautaset T, Winther-Larsen HC, Haugan K, Valla S. (1997) Construction
and use of a versatile set of broad-host-range cloning and expression
vectors based on the RK2 replicon.
Appl Environ Microbiol. 1997 Feb;63(2):370-9.
PMID: 9023917; UI: 97176371
|
| 1997
|
Brautaset,
T., Petersen, S., and Valla, S. (1997) An experimental study in Escherichia
coli of parameters affecting carbon flow in a metabolic pathway.
Biotechnol.
and Bioeng.58: 299-302.
|
| 1997
|
Rehm
BHA, Valla S. (1997) Bacterial alginates: biosynthesis and applications.
Appl Microbiol Biotechnol. 1997 Sep;48(3):281-8. Review.
PMID:
9352672; UI: 98013987
|
| 1997
|
Blatny
JM, Brautaset T, Winther-Larsen HC, Karunakaran P, Valla S. (1997) Improved
broad-host-range RK2 vectors useful for high and low regulated gene expression
levels in gram-negative bacteria.
Plasmid. 1997;38(1):35-51.
PMID:
9281494; UI: 97428562
|
| 1996
|
Rokenes
TP, Lamark T, Strom AR. (1996) DNA-binding properties of the BetI repressor
protein of Escherichia coli: the inducer choline stimulates BetI-DNA complex
formation.
J Bacteriol. 1996 Mar;178(6):1663-70.
PMID: 8626295;
UI: 96198170
|
| 1996
|
Valla, S, and Skjåk-Bræk, G. (1996) Alginate
- a target molecule for genetic engineers and a versatile material for
the biotechnologist.
Agro Food Industry hi-tech. Sept./Oct. issue page
38-41.
|
| 1996
|
Rehm
BH, Ertesvag H, Valla S. (1996) A new Azotobacter vinelandii mannuronan
C-5-epimerase gene (algG) is part of an alg gene cluster physically organized
in a manner similar to that in Pseudomonas aeruginosa.
J Bacteriol.
1996 Oct;178(20):5884-9.
PMID: 8830682; UI: 96427318
|
| 1996
|
Lamark
T, Rokenes TP, McDougall J, Strom AR. (1996) The complex bet promoters
of Escherichia coli: regulation by oxygen (ArcA), choline (BetI), and
osmotic stress.
J Bacteriol. Mar;178(6):1655-62.
PMID: 8626294;
UI: 96198169
|
| 1996
|
Ertesvåg, H., Skjåk-Bræk, G., and
Valla, S. (1996) Genetics and biosynthesis of alginates.
Carbohydr.
Res. May issue, page 14-18
|
| 1995
|
Haugan
K, Karunakaran P, Tondervik A, Valla S. (1995) The host range of RK2 minimal
replicon copy-up mutants is limited by species-specific differences in
the maximum tolerable copy number.
Plasmid. 1995 Jan;33(1):27-39.
PMID: 7753906; UI: 95273473
|
| 1995
|
Ertesvag
H, Hoidal HK, Hals IK, Rian A, Doseth B, Valla S. (1995) A family of modular
type mannuronan C-5-epimerase genes controls alginate structure in Azotobacter
vinelandii.
Mol Microbiol. 1995 May;16(4):719-31.
PMID: 7476166;
UI: 96065700
|
